The PPSQ Gradient Reagent Kit contains all the solvents required for installation and operation up to 500 cycles. Column, protein and amino acids standards are not included. Contains: PTH Amino Acids Mobile Phase A (5x1L) and B (3x1L), 30% N-methylpiperidine (1x200mL), 25% Trifluoroacetic acid (1x40 mL), 5% phenyl isothiocyanate n-heptane solution (1x40mL), ethyl acetate (5x200mL), 1-chlorobutane (3x200mL), 37% acetonitrile (3x200mL), Trifluoroacetic acid (1x 40mL)