Shim-pack Scepter prep columns consist of an organosilane hybrid particle for excellent stability and performance and reliable scaleup up from a Scepter C4-300 analytical column The C4-300 phase is recommended for mid- to large-sized molecules (above 5000 MW) to ensure retention and avoid size exclusion effects. It is ideal for LC and LCMS analysis of peptides, proteins, intact monoclonal antibodies and their subunits up to 150 kDa, and compatible with mobile phase conditions up to pH 10 and 100% aqueous. USP L26, pore size: 300A, pH range: 1-10, max pressure: 45 MPa/450 bar/6,500 psi, fully endcapped. Method development on an analytical Scepter C4-300 column is highly recommended before purchasing a prep column. Prep columns cannot be returned or exchanged once used. Use with prep guard cartridges 227-31194-03 and holder 227-31171-03 to prolong the life of the column. Similar to XBridge Protein BEH C4.