Validation Kit consists of three columns packed with three different batches of sorbent. Shim-pack Arata LC column series enables you to achieve unprecedented resolution by delivering the advantage of unmatched peak shape of basic compounds. Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for basic compounds. Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds with Shim-pack Arata LC Columns. Even with low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase, excellent peak shape of both basic compounds and acidic compounds could be achieved. In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS (/MS) analysis. Excellent peak shape and separation performance for peptides could be achieved on the Shim-pack Arata LC column even with 0.1 % formic acid (weak ion paring acid) containing mobile phase. When analyzing basic compounds on a typical ODS column with low ionic strength acidic mobile phase, peak shape and long equilibration times are common problems. Shim-pack Arata LC columns can be rapidly equilibrated in low ionic strength acidic mobile phases yielding excellent peak shape and stable retention times.