The Mastro2 column has a biocompatible PEEK-lined stainless steel body with a high pressure polymer frit. This inertness eliminates adsorption and reduces tailing for compounds that tend to form chelates with metal surfaces, especially those containing phosphate groups. Maximum operating pressure is 50MPa/500 bar/7200 psi. pH: 2 - 7.5. Maximum temperature is 80C (pH 3 or higher.)