The PTFE filter vial has a patented design for easy compression and complete sample filtration. The 0.45uM membrane is recommended for standard HPLC systems with 0.3mm ID tubing to remove sample particulates and prolong the life of system components such as the autosampler needle, injection port and valve, and column inlet tubing. PTFE is recommended for applications including pesticide analysis in foods, drugs and small molecules, and other samples where the diluent contains methanol or acetonitrile. Use with samples that contain less than 10% solid particulates. Refer to the solvent compatibility table for a complete list of recommended solvents. Use in any HPLC or GC autosampler rack that accepts 12x32mm 2mL vials. Needle stroke must be set to 5mm from the bottom of the vial. Requires a minimum sample volume of 250 uL, maximum sample fill volume 450 uL, filter dead volume 120 uL.